Sheep anti-Human Lambda Free Light Chain Antibody Affinity Purified
Product Details
Specifications
By immunoelectrophoresis this antibody reacts specifically with lambda free light chains. No antibody was detected against kappa light chain or other serum proteins. This antibody may cross react with lambda light chain from other species. Antiserum was solid phase adsorbed to ensure specificity. The antiserum was fractionated and passed over DEAE to yield an IgG fraction.
Total protein was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Total Protein: 15 mg/ml
By immunoelectrophoresis this antibody reacts specifically with lambda free light chain in Bence Jones Urines. No antibody was detected against kappa light chains or serum proteins.
Additional Product Information
The lambda light chain is a component of an Ig molecule that typically forms an Ig molecule linking to a heavy chain. The human body makes both kappa and lambda light chains, and elevated lambda levels may suggest disorders such as amyloidosis or multiple myeloma.
Applications
Not all listed applications have been specifically tested by our laboratory.
For use in precipitin gel reactions (e.g. immunoelectrophoresis (IEP), double diffusion Ouchterlony (DD) or immunofixation electrophoresis (IFE)).
Optimal working dilutions should be determined experimentally by the investigator.