Ensuring Antibody Specificity and Reproducibility: The Six Pillars of Antibody Validation
An antibody is only useful if you can trust the data it generates. How do you know that your antibody is truly binding your protein of interest? What if the antibody cross-reacts with non-target proteins? Or if the antibody generates an unexpected banding pattern?
The presence of improperly validated antibodies on the market can make it difficult to answer questions like the ones above. That’s why it is important to select antibodies from manufacturers like Bethyl, that extensively validate their antibodies so you can generate reproducible and trustworthy data.
This white paper is designed to help you understand key concepts in antibody validation, including:
- Antibody validation methods
- Using antibody validation assays to overcome common challenges in antibody-based experiments
- The role of manufacturers and scientists in antibody validation
- Recommendations in selecting antibodies for your experiment
