Application Note: Flow Cytometry Protocols for Extracellular & Intracellular Targets
Flow Cytometry Protocols for Extracellular & Intracellular Targets – Download this application note to learn about the basics of flow cytometry assay, fluorochrome labels selection, and the protocols for intracellular and extracellular targets.
Flow cytometry is a high throughput quantitative analysis tool that employs fluorescent-labeled molecules for multi-parametric analysis of millions of individual cells or particles. Flow cytometric analysis provides data about the size and granularity and can also be used for the isolation of a particular cell type from a heterogeneous mix of cell populations. In biomedical sciences, the flow cytometry assays involve a careful selection of antibodies and fluorochrome labels and often require optimization of protocols for an accurate analysis of sample-specific target antigens.
Download this application note to learn about:
- Basics of Flow Cytometry Application
- Extracellular and Intracellular Protein Targets
- Direct and Indirect Detection Methods
- Protocols for Extracellular and Intracellular Targets
