RNase Inhibitor, Murine
Improved Heat and Oxidation Resistance
RNA molecules are extremely sensitive to ribonucleases (RNases), which are ubiquitous in the environment and can rapidly degrade RNA molecules, leading to erroneous experimental results. Fortis’ murine RNase inhibitor inhibits RNases A, B, and C through a high-affinity, noncovalent interaction. It can be added directly to reverse transcription reactions or to one-step RT-PCR reactions alongside other reaction components. RNase inhibitor does not inhibit activity of Taq DNA polymerase, T7 RNA polymerase, or any of several commonly used reverse transcriptases including those derived from MMLV, AMV, or HIV. This ensures preservation of high-quality RNA samples and accurate experimental results.
Features and Benefits
- Does not inhibit other RNases and reverse transcriptases
- Isolated from recombinant source
- Available in glycerol and glycerol-free versions
- Enzyme concentrations, volumes, and buffer components can be customized for your needs!
Fortis RNase Inhibitor vs. NEB product. NEB Murine RNase Inhibitor (orange) and Fortis Life Sciences RNase Inhibitor (blue) is compared in an RT-PCR assay using primers and probe specific to Smooth Muscle RNA in the presence of RNase A. A positive control reaction containing only RNase A (red) is included to demonstrate complete degradation of Smooth Muscle RNA template. The cycle number is compared to the relative fluorescence intensity. Fortis Life Science’s RNase Inhibitor restores amplification in the presence of RNase A and demonstrates equivalency to NEB Murine RNase Inhibitor.
