RT-qULTRA Master Mix
Probe-based reverse transcription is a widely used technique for RNA detection and quantification that involves converting RNA into complementary DNA (cDNA) and then using fluorescent probes to measure the amount of cDNA produced in a PCR reaction.
Fortis’ One-Step RT-qULTRA PCR Master Mix is formulated for sensitive RNA detection and quantification by probe-based reverse transcription. It contains optimized components that enable cDNA synthesis and PCR amplification to be performed and quantified in a single tube.
Features & Benefits
- Ready to use standard 2X concentration
- Robust buffer components suitable for RNA, cDNA, and DNA templates
- Contains hot-start polymerase with 2 minute activation
- Engineered MMLV-RT with reduced RNase H activity
- Enzyme concentrations, volumes, buffer components, and working concentration can be customized for your needs!
Fortis RT-qULTRA vs. PrimeScript III: Twist Synthetic SARS-CoV-2 CDC N1 gene RNA RT-qPCR performed with 50K, 5K, 500, or 50 total copies of RNA per 20 µL reaction. Cycling conditions 50 °C, 10 minutes; 95 °C, 10 minutes; 95 °C, 10 seconds; 60 °C, 30 seconds; Repeat 4–5 × 45 cycles. RT-qULTRA with and without RNase Inhibitor (RI).
Similar results obtained with the SARS-CoV-2 CDC N2 gene. RT-qULTRA also produced consistent results against Bio-Rad Reliance MasterMix in similar experiments.
