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Custom Multiplex Imaging & Histology CRO Services

Multiplex immunofluorescence (mIF) is a powerful technique that allows researchers to simultaneously visualize and analyze multiple components of the tissue microenvironment. Multiplexing uses multiple antibodies with fluorescent detection to target specific proteins within the microenvironment, allowing researchers to create a detailed map of the various cells, proteins and their interactions. We offer both low plex and high plex options for mIF which allow you to profile tissues to determine cell phenotypes, their functional state, and cell interactions.

Key Service Offerings

  • Core panels encompassing basic human I-O phenotypic markers
  • Up to 40-plex discovery panels
  • Custom validation of antibodies of your choosing
  • Library of validated antibodies to choose from
  • Creation of custom panels
  • Antibodies can be combined with a core panel to create higher plex panels
  • Histology services to prepare tissue for staining

We can support your needs across the entire imaging workflow

Our Equipment

PhenoImager™ HT

Services

  • Up to 6-plex panel using Opal™ reagents
  • Panel optimization and antibody order testing
  • Library of validated antibodies
  • Sample staining
  • Slide scanning

Specs

  • 0.25 microns/pixel
  • Separate 7-colors (DAPI plus 6 antibodies)
  • Capable of large tissue microarrays
  • Low plex, high throughput, 80 slide capacity

Lunaphore COMET™

Services

  • Runs of 40-plex using seqIF™
  • Core Panels available, including SPYRE™ Antibody Panels from Lunaphore
  • Addition of other catalog or custom antibodies to Core Panel
  • Creation of bespoke panels
  • Library of validated antibodies to choose from

Specs

  • 0.23 microns/pixel
  • 9×9 mm scan area can accommodate tissue sections and small tissue arrays
  • No need for conjugated primary antibodies
  • High plex, medium throughput
    • 24 slides/week for 10-plex
    • 20 slides/week for 20-plex
    • 8 slides/week for 40-plex

Modular Panel Design

10× Core Panel for human I-O markers

Marker

Catalog #

Host Species

Relevance

CD3

A700-016

Rb

T-cells

CD20

A500-017A

Mo

B-cells

FoxP3

A700-034

Rb

Regulatory T-cells

CD8

A500-021A

Mo

Cytotoxic T-cells

PD-L1

A700-020

Rb

Checkpoint

Pan-CK

A500-019

Mo

Epithelium

PD-1

A700-076

Rb

T-cells

CD68

A500-018

Mo

Macrophages

CD45

A700-012

Rb

Lymphocytes

PCNA

A500-024A

Mo

Proliferation

EMT Panel

Target

Clone

Relevance

Beta-catenin

BLR086G

Transcription factor

Vimentin

BLR100G

Mesenchymal

ZEB1

BLR217K

Transcription factor

EpCAM

BLR077G

Epithelial

E-cadherin

BLR088G

Epithelial

SOXIO

BLR080G

Mesenchymal

ZO-1/TJP1

BLR092G

Epithelial

ASMA

BLR082G

Epithelial

T-cell Profiling Panel

Target

Clone

Relevance

GATA3

BLR121H

Th2 naive T cell

T-bet

BLRI10H

Th1 naive T cell

CD25

BLR158J

Regulatory T cell; Activated

CD4

BL-155-1C11

Helper cells

CD45RO

UCHL-1

Memory T cells

Granzyme B

BLRO22E

Cytotoxic T cell

Breast Cancer Module

Target

Clone

Relevance

EGFR

BLR252L

tumorigenesis

PR

188

modulates ERa; prognostic marker

HER2

BLR241L

oncogene; prognostic marker

ERa

119-13

prognostic marker; linked with PR

Cytokeratin (Structural) Module

Target

Clone

Relevance

CK7

BC1

Type II, nonkeratinizing epithelia

CK14

LLO02

Type I, stratified epithelium

CK18

LDK18

Type I, simple epithelia

CK19

BA17

Simple and complex epithelium

СК20

BLR105H

Type I, mature enterocytes
  • Extensive collection of in-house manufactured antibodies to choose from to customize your assay
    • See all available antibodies
    • Immune cell markers
    • Cytokeratin markers
    • Cancer markers
    • Phospho-proteins
    • Signaling pathway
    • Neurobiology markers
    • Mouse proteins
  • Assistance with sourcing other antibodies

Full Service Histology Lab

  • Sectioning
  • Tissue microarray
  • H&E staining
  • Whole Slide Scanning

Additional Resources

Custom Request

Fortis Life Sciences is a registered trademark of Fortis Life Sciences, LLC.
COMET, seqIF, and SPYRE are registered trademarks of Lunaphore Technologies S.A.
PhenoImager, Opal, and Akoya Biosciences are registered trademarks of Akoya Biosciences, Inc.