IgG (immunoglobulin G) is a glycoprotein antibody. It works to regulate immune responses whilst also being involved in autoimmune condition development. Mouse IgG is made up of four peptide chains (two light and two heavy chains.) Here, we will examine mouse IgG, its features, and applications.
Explaining IgG
IgG is the most commonly occurring antibody isotype that is found in extracellular fluid and blood, meaning it can manage infection of body tissues. Immunoglobulins are large, Y-shaped proteins that are formed by B-cells, binding a range of pathogens such as bacteria, viruses, and fungi which safeguards cells and stops the body from getting infected.
The production of IgG is one of the key functions of the humoral immune system, with five distinct classes (IgA, IgD, IgE, IgG, and IgM). The purest, best quality immunoglobulins are used in research, medicine, and immunofluorescence.
IgG manages to safeguard cells by using a range of immune mechanisms:
- Binding - this leads to immobilization and agglutination of the pathogens
- Coating - covering the surfaces of pathogens means they can be recognized and ingested by phagocytic immune cells
- Activation - activating the classical pathway of the complement system. This leads to immune protein production and ultimately leads to the destruction of the pathogen and toxin neutralization.
What Makes Mouse IgG so Important?
Most immunoglobulins are known as polymers whilst mouse IgG is made up of four peptide chains and may be described as a monomer. Generally, mouse IgG makes up around 75% serum immunoglobulins which are then synthesized and secreted by plasma B cells. Mouse IgG is particularly well suited to flow cytometry, western blot, immunohistochemistry applications, and immunoprecipitation, owing to its perfect negative control.
What is Anti Mouse IgG?
Anti-mouse secondary antibodies such as IgG are created via the immunization of sizing host animals with a population of mouse immunoglobulin. When the host animal has an immune response to the foreign body it creates anti-Mouse IgG. Once these are produced the serum is collected and target antibodies purified.
The Mouse IgG ELISA Kit from Fortis Life Sciences
At Fortis Life Sciences, we are well-equipped to deal with any issues relating to Mouse IgG. Inside the ELISA kit are pre-matched antibody pairs, reagents, pairs that can be used for quantitative enzymes. The enzyme-linked immunosorbent assays identify and quantify the levels of protein in a Mouse IgG.
To find out more about our anti-mouse IgG ELISA kit, get in touch with the team today.
