Bethyl Laboratories
Rabbit anti-PSMB7 Antibody Affinity Purified
Primary
Catalog #A303-848A
TargetPSMB7
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, WB
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Product Description
PSMB7 [proteasome (prosome, macropain) subunit, beta type,7] is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit [taken from NCBI Entrez Gene (Gene ID: 5695)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 175 and 225
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPSMB7
Antigen Species
Human
Gene ID5695
Gene Symbol
PSMB7
Gene Name
proteasome subunit beta 7
Uniprot IDQ99436
Protein Name
Proteasome subunit beta type-7
Alternate Names
epididymis secretory sperm binding protein • macropain chain Z • multicatalytic endopeptidase complex chain Z • proteasome (prosome, macropain) subunit, beta type, 7 • proteasome catalytic subunit 2 • proteasome subunit alpha • proteasome subunit beta type-7 • proteasome subunit Z • protein serine kinase c17 • Z
Product Information
Applications
IHC, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PSMB7 immobilized on solid support.
The epitope recognized by A303-848A maps to a region between residue 150 and 200 of human Proteasome (Prosome, Macropain) Subunit, Beta Type, 7 using the numbering given in entry NP_002790.1 (GeneID 5695).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-848A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-848A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-848AOpen
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