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Custom VHH (Single-Domain Antibody) Discovery Services

Conventional antibodies are one of the most widely used research tools in the biopharma industry. Yet despite their popularity, limitations in targeted therapeutics exist – due to both their structure and size, prohibiting their ability to stably reach target epitopes.

VHH antibodies can help overcome these challenges and are quickly becoming a promising tool in targeted therapeutic discovery and development.


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Custom Nanobody Discovery Services

What are Single-Domain Antibodies?

Single-domain antibodies (also known as VHH) are small, ~15kDa fragments isolated from a special type of heavy chain-only antibody that is produced by camelids, such as llamas and alpacas.

The Fortis Life Sciences® Difference

  • High Quality Products
  • Leading Technical Support
  • Custom Services to Meet Your Specific Requirements

Our vertically integrated single-domain antibody discovery process begins with immunization of llamas and alpacas. Proprietary methods are used to ensure the VHH region is amplified and cloned into the phagemid for phage display panning and screening for VHH hits, resulting in complete sequences of your custom VHH library or recombinant expression of the single-domain antibody.

Why Work With Us:

High Quality Cells and RNA:

We strive to preserve cell health for RNA isolation. Animal blood is processed quickly to purify peripheral mononuclear cells. RNA is double-purified with phenol-chloroform extraction and spin columns and examined by electrophoresis prior to making cDNA to ensure cell quality meets our standards before generation of the cDNA library.

Unique PCR Primers:

We perform extensive bioinformatics research on VHH genes and design novel primers for RT-PCR to ensure we amplify the VHH cDNA from camelid antibodies and not conventional antibodies. VHH libraries constructed with these primers have higher diversity and larger coverage of VHH repertoires.

Robust Independent Clones:

We deliver libraries with large numbers of independent clones. Our PBMC isolation and library construction protocol routinely yields >1×108 PBMC cells from each production bleed.

Flexible Deliverables:

Whether you need multiple libraries sequenced, one of four different panning methods including cell panning, production of single-domain antibodies, and other unique requirements for your project, we work with you to make sure our deliverables meet your needs.

Resources

Custom Single-Domain Antibody Discovery Services FAQ

Top 5 Clinical Applications for Llama Antibodies - Abcore

Contact us today to learn how single-domain antibodies can help you advance your research.

*Abcore is now a Fortis Life Sciences Company, read more here.