Rabbit anti-EG5/KIF11 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
The kinesin superfamily of proteins consists of forty five KIF motor proteins that function in intracellular transport along microtubules. Kinesin activity has been linked to various cellular functions such as vesicle transport, mitotic spindle formation, chromosome segregation, and cytokinesis. Structurally, all kinesins contain a motor domain with microtubule and nucleotide binding sites that utilize ATP to target cargo along microtubule filaments. EG5/KIF11 plays a role in spindle-pole formation and chromosome positioning and separation.
Alternate Names
EG5; HKSP; kinesin-like protein 1; kinesin-like protein KIF11; kinesin-like spindle protein HKSP; kinesin-related motor protein Eg5; KNSL1; MCLMR; thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP5; TRIP-5
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.