NEW!

Rabbit anti-PD-L1 Recombinant Monoclonal Antibody [BLR020E]

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
PD-L1
Reactivity:
Human
Platforms:
COMET™, PhenoImager™ HT
Host:
Rabbit
Clonality:
Recombinant Monoclonal
Clone:
BLR020E
Format:
Whole IgG
Immunogen:
Between 240 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Purified
100 µl (50+ tests)

10 µl (5+ tests)

100 µg (BSA-Free)

$127.00 $794.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
50 µg/ml 1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Request Formulation Change
Production Procedures
Recombinant antibody was purified from cell culture supernatant.

Immunogen was a synthetic peptide representing a region between residue 250 and the C-terminus (residue 290) of human programmed cell death 1 ligand 1 (PD-L1) according to the numbering given in entry NP_054862.1 (GeneID 29126).
Country of Origin
USA

Additional Product Information

PD-L1 is an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. PD-L1 is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma [taken from NCBI Entrez Gene (Gene ID: 55193)].

29126
CD274
CD274 molecule
Q9NZQ7
Programmed cell death 1 ligand 1

Alternate Names

B7 homolog 1; B7-H; B7H1; B7-H1; CD antigen CD274; CD274 antigen; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; programmed cell death 1 ligand 1; Programmed death ligand 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.