Rabbit anti-E-Cadherin Recombinant Monoclonal Antibody [BLR088G]
Product Details
Specifications
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
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Immunogen was a recombinant protein representing the extracellular domain of human E-Cadherin (residues 155-707, NP_004351.1).
Additional Product Information
Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in adjacent cells. E-Cadherin, or Cadherin-1, is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells and has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P12830].
Alternate Names
Arc-1; BCDS1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD antigen CD324; CD324; CDHE; cell-CAM 120/80; ECAD; E-cadherin; E-cadherin 1; epididymis secretory sperm binding protein; epithelial cadherin; LCAM; UVO; uvomorulin
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.