Rabbit anti-GATA3 Recombinant Monoclonal Antibody [BLR121H]
Product Details
Specifications
Immunogen was a peptide representing a region between residue 150 and 200 of human trans-acting T-cell-specific transcription factor GATA3 using the numbering given in entry NM_001002295.1 (Gene ID 2625).
Additional Product Information
GATA binding protein 3 (GATA3) is a transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
Alternate Names
GATA-binding factor 3; HDR; HDRS; trans-acting T-cell-specific transcription factor GATA-3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.