Rabbit anti-Histone H4 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog # A300-647A
Validated
Target:
Histone H4
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 53 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

10 µl (200 µg/ml)

A300-647A
$464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Mouse, Chicken, Pig, X. laevis, X. tropicalis, Bovine, Rat, D. melanogaster
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Request Formulation Change
Production Procedures
Antibody was affinity purified using an epitope specific to Histone H4 immobilized on solid support.

The epitope recognized by A300-647A maps to a region between residue 50 and the C-terminus (residue 103) of human Histone H4 using the numbering given in entry NP_778224.1 (GeneID 121504).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

Histone H4 is one of four histone proteins that assemble into an octamer to form the nucleosome core and serve to spool and compact DNA into chromatin. Histones are subject to a complex set of post-translational modifications (acetylation, methylation, phosphorylation, ubiquitination, sumoylation, citrullination, and ADP ribosylation) that are important to the regulation of gene transcription, DNA replication, and DNA repair.

121504
HIST4H4
histone cluster 4 H4
P62805
Histone H4

Alternate Names

H4/p; histone 4, H4; histone H4

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.

Immunohistochemistry (IHC)
1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)
6 µg/mg lysate
Western Blot (WB)
1:2000 - 1:10,000