Rabbit anti-Histone H4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-647A maps to a region between residue 50 and the C-terminus (residue 103) of human Histone H4 using the numbering given in entry NP_778224.1 (GeneID 121504).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Histone H4 is one of four histone proteins that assemble into an octamer to form the nucleosome core and serve to spool and compact DNA into chromatin. Histones are subject to a complex set of post-translational modifications (acetylation, methylation, phosphorylation, ubiquitination, sumoylation, citrullination, and ADP ribosylation) that are important to the regulation of gene transcription, DNA replication, and DNA repair.
Alternate Names
H4/p; histone 4, H4; histone H4
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.