Rabbit anti-Insulin Receptor Beta Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
The insulin receptor is a receptor tyrosine kinase that mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates to stimulate uptake of glucose. The insulin receptor is synthesized as a single polypeptide that is cleaved to generate an alpha and beta subunit. The subunit structure of the insulin receptor consists of a tetramer of two alpha and two beta chains linked by disulfide bonds.
Alternate Names
CD antigen CD220; CD220; HHF5; insulin receptor; IR
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.