Rabbit anti-WDR77/MEP50 IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00338 maps to a region between residue 292 and 342 of human WD repeat domain 77 (methylosome protein 50) using the numbering given in entry NP_077007.1 (GeneID 79084).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to WDR77/MEP50 immobilized on solid support.
The epitope recognized by IHC-00338-T maps to a region between residue 292 and 342 of human WD repeat domain 77 (methylosome protein 50) using the numbering given in entry NP_077007.1 (GeneID 79084).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
WDR77/MEP50 is a component of the methylosome, a large protein arginine methyltransferase complex that modifies specific arginines to dimethylarginines in splicesomal Sm proteins. WDR77/MEP50 associates with the methyltransferase JBP1 (PRMT5) and is important for methylosome activity. WDR77/MEP50 has also been shown to associate with the polycomb group protein, SUZ12, and histone H2A. In this context, WDR77/MEP50 is proposed to function as an adaptor between PRMT5 and its substrate H2A to regulate transcription.
Alternate Names
androgen receptor cofactor p44; HKMT1069; MEP50; MEP-50; methylosome protein 50; Nbla10071; p44; p44/Mep50; testis tissue sperm-binding protein Li 44a; WD repeat-containing protein 77
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.