Rabbit anti-hSET1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-290A maps to a region between residue 1675 and the C-terminus (residue 1709) of human hSET1 (KIAA0339) using the numbering given in TrEMBL entry O15047 (GeneID 9739).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to hSET1 immobilized on solid support.
The epitope recognized by A300-290A-T maps to a region between residue 1675 and the C-terminus (residue 1709) of human hSET1 (KIAA0339) using the numbering given in TrEMBL entry O15047 (GeneID 9739).
Additional Product Information
hSET1 is a subunit of a human Set1-like histone methyltransferase (HMT) complex. The Set-1 like complexes are responsible for mono-, di-, and tri-methylation of histone H3 K4. The Set class of proteins takes its name from the 130-140 aa motif SET domain identified in drosophila proteins. hSET1 associates with a multitude of proteins in a Set1-like complex which includes hSet1, Ash2L, RBBP5,WDR5, hDPY-30, hSwd2, CXXC1, and HCF1.
Alternate Names
histone-lysine N-methyltransferase SETD1A; hSET1A; KMT2F; lysine N-methyltransferase 2F; SET domain-containing protein 1A; Set1; set1/Ash2 histone methyltransferase complex subunit SET1; Set1A
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.