Rabbit anti-eIF2alpha/EIF2S1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-721A maps to a region between residue 275 and the C-terminus (residue 315) of human Eukarytic Translation Initiation Factor 2, subunit 1 alpha, 35kDa using the numbering given in entry NP_004085.1 (GeneID 1965). Antibody was affinity purified using an epitope specific to eIF2alpha/EIF2S1 immobilized on solid support.
The epitope recognized by A300-721A-T maps to a region between residue 275 and the C-terminus (residue 315) of human Eukarytic Translation Initiation Factor 2, subunit 1 alpha, 35kDa using the numbering given in entry NP_004085.1 (GeneID 1965).
Additional Product Information
eIF-2alpha is the alpha subunit of the eukaryotic initiation factor 2. The EIF2 forms a ternary complex with GTP and initiator Met-tRNAi and is responsible for the binding of initiator Met-tRNAi and mRNA to 40S ribosomes for protein synthesis.
Alternate Names
EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.