Rabbit anti-SRA Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-743A maps to a region between residue 180 and the C-terminus (residue 237) of human Steroid Receptor RNA Activator 1 using the numbering given in entry NP_001030312.1 (GeneID 10011).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SRA immobilized on solid support.
The epitope recognized by A300-743A-T maps to a region between residue 180 and the C-terminus (residue 237) of human Steroid Receptor RNA Activator 1 using the numbering given in entry NP_001030312.1 (GeneID 10011).
Additional Product Information
Steroid receptor RNA activator 1 (SRA) was originally identified as a functional non-coding RNA that has been shown to be a coactivator of steroid receptors for androgens (AR), estrogens (ER), glucocorticoids, and progestins (PR). SRA exists in ribonucleoprotein complexes with steroid receptor coactivators (SRCs) and steroid receptors and may modulate transcriptional specificity. A 236 amino-acid protein encoded by an SRA-RNA isoform has also been identified. The SRA protein (SRAP) is widely expressed in breast cancer cell lines and breast cancer and has been demonstrated to modify the activity of the estrogen receptor.
Alternate Names
pp7684; SRA; SRAP; steroid receptor coactivator; steroid receptor RNA activator 1; steroid receptor RNA activator 1 (complexes with NCOA1); steroid receptor RNA activator protein; STRAA1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.