Rabbit anti-NCOA62 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-785A maps to a region between residue 500 and the C-terminus (residue 536) of human Nuclear Receptor Coactivator, 62 kD (SKIIP, SKI Interacting Protein) using the numbering given in entry NP_036377.1 (GeneID 22938).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NCOA62 immobilized on solid support.
The epitope recognized by A300-785A-T maps to a region between residue 500 and the C-terminus (residue 536) of human Nuclear Receptor Coactivator, 62 kD (SKIIP, SKI Interacting Protein) using the numbering given in entry NP_036377.1 (GeneID 22938).
Additional Product Information
NCOA62 is an integral component of the splicesome that functions in pre-mRNA splicing. NCOA62 has also been demonstrated to function as a coactivator of the Notch transcription factor and the vitamin D nuclear receptor. Additionally, NCOA2 exhibits transcriptional repression activity and associates with the N-Cor and SMRT corepressors. Recent evidence suggests a role for NCOA62 in transcription elongation.
Alternate Names
Bx42; FUN20; homolog of Drosophila BX42; NCOA-62; nuclear protein SkiP; nuclear receptor coactivator NCoA-62; nuclear receptor coactivator, 62-kD; Prp45; PRPF45; SKI interacting protein; ski-interacting protein; SKIIP; SKIP; SKIP1; SNW domain-containing protein 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.