Rabbit anti-Pescadillo Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-903A maps to a region between residue 535 and the C-terminus (residue 588) of human Pescadillo using the numbering given in entry NP_055118.1 (GeneID 23481).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Pescadillo immobilized on solid support.
The epitope recognized by A300-903A-T maps to a region between residue 535 and the C-terminus (residue 588) of human Pescadillo using the numbering given in entry NP_055118.1 (GeneID 23481).
Additional Product Information
Pescadillo is a BRCT (BRCA1 C-terminal) domain protein and plays a role in DNA synthesis, ribosome genesis, cell proliferation, and transformation.
Alternate Names
NOP7; PES; pescadillo homolog; pescadillo homolog 1, containing BRCT domain
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.