Rabbit anti-NUFIP2/82-FIP Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-599A maps to a region between residue 550 and 600 of human nuclear fragile X mental retardation protein interacting protein 2 (82 kDa FMRP-interacting protein) using the numbering given in entry NP_065823.1 (GeneID 57532).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NUFIP2/82-FIP immobilized on solid support.
The epitope recognized by A301-599A-T maps to a region between residue 550 and 600 of human nuclear fragile X mental retardation protein interacting protein 2 (82 kDa FMRP-interacting protein) using the numbering given in entry NP_065823.1 (GeneID 57532).
Additional Product Information
NUFIP2/82-FIP is a FMRP (fragile X mental retardation protein)-interacting protein and a component of FMRP-mRNP associated complexes. FMRP is a RNA binding protein that is thought to play role in posttranslational regulation and is the cause of fragile X syndrome.
Alternate Names
182-FIP; 82 kDa FMRP-interacting protein; 82-FIP; 82-kD FMRP Interacting Protein; cell proliferation-inducing gene 1 protein; FIP-82; FMRP-interacting protein 2; Fragile X Mental Retardation Protein; nuclear fragile X mental retardation protein interacting protein 2; nuclear fragile X mental retardation-interacting protein 2; NUFIP2, FMR1 interacting protein 2; PIG1; proliferation-inducing gene 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.