Rabbit anti-eIF3J/EIF3S1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-746A maps to a region between residue 208 and 258 of human eukaryotic translation initiation factor 3, subunit J (eukaryotic translation initiation factor 3, subunit 1) using the numbering given in entry NP_003749.2 (GeneID 8669).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to eIF3J/EIF3S1 immobilized on solid support.
The epitope recognized by A301-746A-T maps to a region between residue 208 and 258 of human eukaryotic translation initiation factor 3, subunit J (eukaryotic translation initiation factor 3, subunit 1) using the numbering given in entry NP_003749.2 (GeneID 8669).
Additional Product Information
Eukaryotic initiation factor 3 subunit J (eIF3J) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5’-end of cellular mRNAs near the cap structure (m7GpppN). eIF3J is proposed to act as a factor that further stabilizes the interaction between eIF3 and the ribosome.
Alternate Names
eIF3 p35; eIF-3-alpha; eIF3-alpha; eIF3j; eIF3-p35; EIF3S1; Eukaryotic translation initiation factor 3 subunit 1; eukaryotic translation initiation factor 3 subunit J; eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD)
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.