Rabbit anti-eIF3D/EIF3S7 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-758A-T maps to a region between residue 150 and 200 of human eukaryotic translation initiation factor 3, subunit D (eukaryotic translation initiation factor 3, subunit 7) using the numbering given in entry NP_003744.1 (GeneID 8664). Antibody was affinity purified using an epitope specific to eIF3D/EIF3S7 immobilized on solid support.
The epitope recognized by A301-758A maps to a region between residue 150 and 200 of human eukaryotic translation initiation factor 3, subunit D (eukaryotic translation initiation factor 3, subunit 7) using the numbering given in entry NP_003744.1 (GeneID 8664).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Eukaryotic initiation factor 3 subunit D (eIF3D) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5’-end of cellular mRNAs near the cap structure (m7GpppN).
Alternate Names
eIF3 p66; eIF3d; eIF3-p66; EIF3S7; eIF-3-zeta; eIF3-zeta; Eukaryotic translation initiation factor 3 subunit 7; eukaryotic translation initiation factor 3 subunit D; eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa; translation initiation factor eIF3 p66 subunit
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.