Rabbit anti-RIOK2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-450A maps to a region between residue 502 and 552 of human RIO kinase 2 using the numbering given in entry NP_060813.1 (GeneID 55781).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RIOK2 immobilized on solid support.
The epitope recognized by A302-450A-T maps to a region between residue 502 and 552 of human RIO kinase 2 using the numbering given in entry NP_060813.1 (GeneID 55781).
Additional Product Information
RIO kinase 2 (RIOK2) is a member of the RIO family of atypical serine protein kinases first characterized in yeast. There are three RIO kinase subfamilies: RIO1, RIO2, and RIO3. RIOK1 and RIOK2 proteins are present in organisms from Archaea to humans. Studies of RIO kinases in yeast indicate that they play a role in ribosome biogenesis. Human RIOK2 has been shown to be a part of a late 40S pre-ribosomal particle and have influence on 40S maturation.
Alternate Names
RIO kinase 2; RIO2; serine/threonine-protein kinase RIO2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.