Rabbit anti-RECQ5 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-522A maps to a region between residue 650 and 700 of human RecQ protein-like 5 using the numbering given in entry NP_004250.4 (GeneID 9400).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RECQ5 immobilized on solid support.
The epitope recognized by A302-522A-T maps to a region between residue 650 and 700 of human RecQ protein-like 5 using the numbering given in entry NP_004250.4 (GeneID 9400).
Additional Product Information
RECQ5 is one of five human homologs of RecQ DNA helicases. RecQ helicases function in DNA replication and repair and are important to genomic stability. Mutations in the genes encoding BLM (RECQL2), WRN (RECQL3), and RECQL4 result in a predisposition to cancer. No heritable disease has been found to be associated with RECQL5.
Alternate Names
ATP-dependent DNA helicase Q5; DNA helicase, RecQ-like type 5; RecQ helicase-like 5; RecQ protein-like 5; RECQ5
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.