Rabbit anti-Arpc1b Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-781A maps to a region between residue 322 and 372 of human Actin Related Protein 2/3 complex, Subunit 1B using the numbering given in entry NP_005711.1 (GeneID 10095).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Arpc1b immobilized on solid support.
The epitope recognized by A302-781A-T maps to a region between residue 322 and 372 of human Actin Related Protein 2/3 complex, Subunit 1B using the numbering given in entry NP_005711.1 (GeneID 10095).
Additional Product Information
Arpc1b (actin-related protein 2/3 complex subunit 1B) functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Recently Arpc1b has been found to localize at centrosomes and function in centrosome homeostasis.
Alternate Names
actin related protein 2/3 complex, subunit 1B, 41kDa; actin-related protein 2/3 complex subunit 1B; ARC41; arp2/3 complex 41 kDa subunit; ARP2/3 protein complex subunit p41; p40-ARC; p41-ARC; PLTEID
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.