Rabbit anti-API5 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-784A maps to a region between residue 1 and 50 of human Apoptosis Inhibitor 5 using the numbering given in entry XP_508375.2 (GeneID 8539).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to API5 immobilized on solid support.
The epitope recognized by A302-784A-T maps to a region between residue 1 and 50 of human Apoptosis Inhibitor 5 using the numbering given in entry XP_508375.2 (GeneID 8539).
Additional Product Information
API5 (apoptosis inhibitor 5) is an antiapoptotic factor that may have a role in protein assembly. API5 negatively regulates ACIN1. By binding to ACIN1, API5 suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. API5 is also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
Alternate Names
AAC11; AAC-11; antiapoptosis clone 11 protein; API-5; apoptosis inhibitor 5; cell migration-inducing gene 8 protein; Fibroblast growth factor 2-interacting factor; fibroblast growth factor 2-interacting factor 2; FIF; migration-inducing protein MIG8; Protein XAGL
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.