Rabbit anti-eIF2B delta/EIF2B4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-982A maps to a region between residue 50 and 100 of human Eukaryotic Translation Initiation Factor 2B, Subunit 4 delta, 67kDa using the numbering given in entry NP_001029288.1 (GeneID 8890).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to eIF2B delta/EIF2B4 immobilized on solid support.
The epitope recognized by A302-982A-T maps to a region between residue 50 and 100 of human Eukaryotic Translation Initiation Factor 2B, Subunit 4 delta, 67kDa using the numbering given in entry NP_001029288.1 (GeneID 8890).
Additional Product Information
eIF2B delta is encoded by the EIF2B4 gene. EIF2B4 is necessary for protein synthesis and is one of five subunits of the eIF2B factor which functions as a GTP exchange factor. The protein encoded by this gene, EIF2B4, is the fourth, or delta subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy.
Alternate Names
EIF2B; EIF-2B; eIF-2B GDP-GTP exchange factor subunit delta; EIF2Bdelta; eukaryotic translation initiation factor 2B subunit 4 delta; eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa; translation initiation factor eIF-2b delta subunit; translation initiation factor eIF-2B subunit delta
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.