Rabbit anti-HDGF Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-170A maps to a region between residue 150 and 200 of human Hepatoma-Derived Growth Factor using the numbering given in entry NP_004485.1 (GeneID 3068).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to HDGF immobilized on solid support.
The epitope recognized by A303-170A-T maps to a region between residue 150 and 200 of human Hepatoma-Derived Growth Factor using the numbering given in entry NP_004485.1 (GeneID 3068).
Additional Product Information
Hepatoma-derived growth factor (HDGF) was identified as a mitogen secreted from the human hepatoma cell line Huh-7. HDGF has been found to bind DNA and to function as a transcriptional repressor.
Alternate Names
epididymis secretory sperm binding protein; HDGF; hepatoma derived growth factor; hepatoma-derived growth factor; high mobility group protein 1-like 2; HMG1L2; HMG-1L2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.