Rabbit anti-UIF Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-525A maps to a region between residue 1 and 50 of human UAP56-Interacting Factor using the numbering given in entry NP_115664.2 (GeneID 84248).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to UIF immobilized on solid support.
The epitope recognized by A303-525A-T maps to a region between residue 1 and 50 of human UAP56-Interacting Factor using the numbering given in entry NP_115664.2 (GeneID 84248).
Additional Product Information
UAP56-interacting factor (UIF) is a factor required for mRNA export from the nucleus to the cytoplasm. UIF acts as an adapter that uses the DDX39B/UAP56-NFX1 pathway to ensure efficient mRNA export and delivering to the nuclear pore. UIF associates with spliced and unspliced mRNAs simultaneously with THOC4 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96QD9].
Alternate Names
forty-two-three domain-containing protein 1; protein 40-2-3; UAP56-interacting factor; UIF
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.