Rabbit anti-MPP11 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-722A maps to a region between residue 571 and 621 of human DnaJ (Hsp40) Homolog, Subfamily C, Member 2 using the numbering given in entry NP_055192.1 (GeneID 27000).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MPP11 immobilized on solid support.
The epitope recognized by A303-722A-T maps to a region between residue 571 and 621 of human DnaJ (Hsp40) Homolog, Subfamily C, Member 2 using the numbering given in entry NP_055192.1 (GeneID 27000).
Additional Product Information
M-phase phosphoprotein 11 is a phosphoprotein with a J domain and a Myb DNA-binding domain which localizes to both the nucleus and the cytosol. MPP11 is capable of forming a heterodimeric complex that associates with ribosomes, acting as a molecular chaperone for nascent polypeptide chains as they exit the ribosome. MPP11 was identified as a leukemia-associated antigen and expression of the gene is upregulated in leukemic blasts. Additionally, chromosomal aberrations involving the DNAJC2 gene are associated with primary head and neck squamous cell tumors [taken from NCBI Entrez Gene (Gene ID: 27000)].
Alternate Names
DnaJ (Hsp40) homolog, subfamily C, member 2; dnaJ homolog subfamily C member 2; M-phase phosphoprotein 11; MPHOSPH11; MPP11; ZRF1; ZUO1; zuotin-related factor 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.