Rabbit anti-EXOSC1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-885A maps to a region between residue 145 and 195 of human Exosome Component 1 using the numbering given in entry NP_057130.1 (GeneID 51013).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to EXOSC1 immobilized on solid support.
The epitope recognized by A303-885A-T maps to a region between residue 145 and 195 of human Exosome Component 1 using the numbering given in entry NP_057130.1 (GeneID 51013).
Additional Product Information
EXOSC1 is a core component of the exosome. The mammalian exosome is required for rapid degradation of AU rich element-containing RNAs but not for poly(A) shortening. The association CSL4 with the exosome is mediated by protein-protein interactions with ribosomal RNA-processing protein 42 and ribosomal RNA-processing protein 46 [taken from NCBI Entrez Gene (Gene ID: 51013)].
Alternate Names
3'-5' exoribonuclease CSL4 homolog; CGI-108; CSL4; Csl4p; exosomal core protein CSL4; exosome complex component CSL4; Exosome component 1; homolog of yeast exosomal core protein CSL4; p13; SKI4; Ski4p
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.