Rabbit anti-PNPase Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-917A maps to a region between residue 700 and 750 of human Polynucleotide Phosphorylase 1 using the numbering given in entry NP_149100.2 (GeneID 87178).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PNPase immobilized on solid support.
The epitope recognized by A303-917A-T maps to a region between residue 700 and 750 of human Polynucleotide Phosphorylase 1 using the numbering given in entry NP_149100.2 (GeneID 87178).
Additional Product Information
Polyribonucleotide nucleotidyltransferase 1 (PNPase) is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8TCS8].
Alternate Names
3'-5' RNA exonuclease OLD35; COXPD13; DFNB70; OLD35; old-35; PNPASE; PNPase 1; PNPase old-35; polynucleotide phosphorylase 1; polynucleotide phosphorylase-like protein; polyribonucleotide nucleotidyltransferase 1, mitochondrial
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.