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Rabbit anti-Raver1 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
Raver1
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 556 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to Raver1 immobilized on solid support.

The epitope recognized by A303-939A-T maps to a region between residue 556 and 606 of human Ribonucleoprotein, PTB-Binding using the numbering given in entry Q8IY67.1 (GeneID 125950).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to Raver1 immobilized on solid support.

The epitope recognized by A303-939A maps to a region between residue 556 and 606 of human Ribonucleoprotein, PTB-Binding using the numbering given in entry Q8IY67.1 (GeneID 125950).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

Raver1 cooperates with PTBP1 to modulate regulated alternative splicing events. Raver1 promotes exon skipping and cooperates with PTBP1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA (by similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8IY67].

125950
RAVER1
ribonucleoprotein, PTB binding 1
Q8IY67
Ribonucleoprotein PTB-binding 1

Alternate Names

protein raver-1; ribonucleoprotein PTB-binding 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.