Rabbit anti-SRP68 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-955A maps to a region between residue 1 to 50 of human Signal Recognition Particle 68kDa using the numbering given in entry NP_055045.2 (GeneID 6730).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SRP68 immobilized on solid support.
The epitope recognized by A303-955A-T maps to a region between residue 1 to 50 of human Signal Recognition Particle 68kDa using the numbering given in entry NP_055045.2 (GeneID 6730).
Additional Product Information
Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. Signal recognition particle 68kDa (SRP68) binds the 7S RNA, SRP72 binds to this complex subsequently. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9UHB9].
Alternate Names
signal recognition particle 68 kDa protein; signal recognition particle 68kD; signal recognition particle 68kDa; signal recognition particle subunit SRP68; SRP68
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.