Rabbit anti-Staufen1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-956A maps to a region between residue 1 to 50 of human Staufen, RNA Binding Protein, Homolog 1 using the numbering given in entry NP_059347.2 (GeneID 6780).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Staufen1 immobilized on solid support.
The epitope recognized by A303-956A-T maps to a region between residue 1 to 50 of human Staufen, RNA Binding Protein, Homolog 1 using the numbering given in entry NP_059347.2 (GeneID 6780).
Additional Product Information
Staufen1 is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. Staufen1 contains a microtubule- binding domain similar to that of microtubule-associated protein 1B, and binds tubulin. Staufen1 has been shown to be present in the cytoplasm in association with the rough endoplasmic reticulum (RER), implicating this protein in the transport of mRNA via the microtubule network to the RER, the site of translation [taken from NCBI Entrez Gene (Gene ID: 67803)].
Alternate Names
double-stranded RNA-binding protein Staufen homolog 1; PPP1R150; protein phosphatase 1, regulatory subunit 150; STAU; staufen, RNA binding protein, homolog 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.