Rabbit anti-EDC3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-986A maps to a region between residue 25 to 75 of human Enhancer of mRNA Decapping 3 using the numbering given in entry NP_079359.2 (GeneID 80153).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to EDC3 immobilized on solid support.
The epitope recognized by A303-986A-T maps to a region between residue 25 to 75 of human Enhancer of mRNA Decapping 3 using the numbering given in entry NP_079359.2 (GeneID 80153).
Additional Product Information
Enhancer of mRNA-decapping protein 3 (EDC3) binds single-stranded RNA. In the process of mRNA degradation, EDC3 may play a role in mRNA decapping. It may play a role in spermiogenesis and oogenesis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96F86].
Alternate Names
enhancer of mRNA decapping 3 homolog; enhancer of mRNA-decapping protein 3; hYjeF_N2; hYjeF_N2-15q23; LSM16; LSM16 homolog; LSM16 homolog (EDC3, S. cerevisiae); MRT50; YJDC; yjeF domain containing; yjeF domain-containing protein 1; yjeF N-terminal domain-containing protein 2; YjeF_N2; YJEFN2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.