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Rabbit anti-RPS14 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
RPS14
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 101 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Presumed Reactivity
Rat, D. melanogaster
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to RPS14 immobilized on solid support.

The epitope recognized by A304-031A maps to a region between residue 101 to 151 of human Ribosomal Protein S14 using the numbering given in entry NP_001020242.1 (GeneID 6208).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to RPS14 immobilized on solid support.

The epitope recognized by A304-031A-T maps to a region between residue 101 to 151 of human Ribosomal Protein S14 using the numbering given in entry NP_001020242.1 (GeneID 6208).
Country of Origin
USA

Additional Product Information

Ribosomal protein S14 (RPS14) belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm [taken from NCBI Entrez Gene (Gene ID: 6208)].

6208
RPS14
ribosomal protein S14
P62263
40S ribosomal protein S14

Alternate Names

40S ribosomal protein S14; emetine resistance; EMTB; S14; small ribosomal subunit protein uS11

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.