Rabbit anti-RNASEH2A Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-149A maps to a region between residue 250 to 300 of human Ribonuclease H2, Subunit A using the numbering given in entry NP_006388.2 (GeneID 10535).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RNASEH2A immobilized on solid support.
The epitope recognized by A304-149A-T maps to a region between residue 250 to 300 of human Ribonuclease H2, Subunit A using the numbering given in entry NP_006388.2 (GeneID 10535).
Additional Product Information
RNASEH2A is the catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. RNASEH2A participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. It also mediates the excision of single ribonucleotides from DNA:RNA duplexes [taken from NCBI Entrez Gene (Gene ID: 10535)].
Alternate Names
AGS4; aicardi-Goutieres syndrome 4 protein; JUNB; ribonuclease H2 subunit A; ribonuclease H2, large subunit; ribonuclease HI large subunit; ribonuclease HI subunit A; RNase H(35); RNase H2 subunit A; RNase HI large subunit; RNASEHI; RNHIA; RNHL; THSD8
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.