Rabbit anti-TARS Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-755A maps to a region between residue 1 to 50 of human Threonyl-tRNA Synthetase using the numbering given in entry P26639.3 (GeneID 6897).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TARS immobilized on solid support.
The epitope recognized by A304-755A-T maps to a region between residue 1 to 50 of human Threonyl-tRNA Synthetase using the numbering given in entry P26639.3 (GeneID 6897).
Additional Product Information
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Threonyl-tRNA synthetase (TARS) belongs to the class-II aminoacyl-tRNA synthetase family [taken from NCBI Entrez Gene (Gene ID: 6897)].
Alternate Names
threonine tRNA ligase 1, cytoplasmic; threonine--tRNA ligase, cytoplasmic; Threonyl-tRNA synthetase; threonyl-tRNA synthetase, cytoplasmic; ThrRS
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.