Rabbit anti-alpha Actinin 4/ACTN4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-768A maps to a region between residue 1 to 50 of human Alpha-actinin-4 using the numbering given in entry O43707 (GeneID 81).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to alpha Actinin 4/ACTN4 immobilized on solid support.
The epitope recognized by A304-768A-T maps to a region between residue 1 to 50 of human Alpha-actinin-4 using the numbering given in entry O43707 (GeneID 81).
Additional Product Information
Alpha Actinin 4 / ACTN4 is an F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). It is probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). It is involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. It links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). alpha Actinin 4 / ACTN4 may also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O43707].
Alternate Names
ACTININ-4; alpha-actinin-4; focal segmental glomerulosclerosis 1; FSGS; FSGS1; non-muscle alpha-actinin 4
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.