Rabbit anti-APEH/AARE Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-808A maps to a region between residue 150 to 200 of human Acylamino-acid-releasing enzyme using the numbering given in entry P13798.4 (GeneID 327).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to APEH/AARE immobilized on solid support.
The epitope recognized by A304-808A-T maps to a region between residue 150 to 200 of human Acylamino-acid-releasing enzyme using the numbering given in entry P13798.4 (GeneID 327).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
APEH catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P13798].
Alternate Names
AARE; ACPH; acylamino-acid-releasing enzyme; acylaminoacyl-peptidase; acyl-peptide hydrolase; APH; D3F15S2; D3S48E; DNF15S2; N-acylaminoacyl-peptide hydrolase; OPH; oxidized protein hydrolase
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).