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Rabbit anti-RPL29/ Ribosomal Protein L29 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
RPL29/Ribosomal Protein L29
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 109 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to RPL29/ Ribosomal Protein L29 immobilized on solid support.

The epitope recognized by A305-056A maps to a region between residue 109 to 159 of human 60S ribosomal protein L29 using the numbering given in entry P47914.2 (GeneID 6159).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to RPL29/ Ribosomal Protein L29 immobilized on solid support.

The epitope recognized by A305-056A-T maps to a region between residue 109 to 159 of human 60S ribosomal protein L29 using the numbering given in entry P47914.2 (GeneID 6159).
Country of Origin
USA

Additional Product Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL29 is a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29E family of ribosomal proteins. The protein is also a peripheral membrane protein expressed on the cell surface that directly binds heparin [taken from NCBI Entrez Gene (Gene ID: 6159)].

6159
RPL29
ribosomal protein L29
P47914
60S ribosomal protein L29

Alternate Names

60S ribosomal protein L29; cell surface heparin-binding protein HIP; heparin/heparan sulfate-binding protein; heparin/heparan sulfate-interacting protein; HIP; HP/HS-interacting protein; HUMRPL29; L29; large ribosomal subunit protein eL29; ribosomal protein YL43 homologue; RPL29_3_370; RPL29P10

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.