Rabbit anti-FSCN1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-240A maps to a region between residue 25 to 75 of human Fascin using the numbering given in entry Q16658.3 (GeneID 6624).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to FSCN1 immobilized on solid support.
The epitope recognized by A305-240A-T maps to a region between residue 25 to 75 of human Fascin using the numbering given in entry Q16658.3 (GeneID 6624).
Additional Product Information
Fascin organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Fascin plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. It is important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q16658].
Alternate Names
55 kDa actin-bundling protein; epididymis secretory sperm binding protein; FAN1; fascin; fascin homolog 1, actin-bundling protein; HSN; p55; singed-like (fascin homolog, sea urchin); Singed-like protein; SNL
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.