Rabbit anti-RNH1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-356A maps to a region between residue 1 to 50 of human Ribonuclease inhibitor using the numbering given in entry P13489.2 (GeneID 6050).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RNH1 immobilized on solid support.
The epitope recognized by A305-356A-T maps to a region between residue 1 to 50 of human Ribonuclease inhibitor using the numbering given in entry P13489.2 (GeneID 6050).
Additional Product Information
Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo [taken from NCBI Entrez Gene (Gene ID: 6050)].
Alternate Names
placental ribonuclease inhibitor; placental RNase inhibitor; RAI; ribonuclease inhibitor; Ribonuclease/angiogenin inhibitor 1; RNH; testicular tissue protein Li 164
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.