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Purified Bovine IgG
Bethyl Laboratories
Catalog #
Target:
Bovine IgG
Host:
Bovine
Purity:
Purified
Product Details
Specifications
Antigen Species
Bovine
Concentration
1 mg/ml
5 mg/ml
Storage
2 - 8 °C
Shelf Life
2 years from date of receipt
Physical State
Liquid
Buffer
Production Procedures
Purified normal bovine IgG is isolated from bovine serum using fractionation and DEAE chromatography.
Protein concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis the IgG was shown to 1) react with antiserum specific for bovine IgG and the bovine subclasses IgG1 and IgG2, 2) not react with antiserum specific for IgA or IgM, and 3) produce a single precipitin arc with antiserum against bovine serum identical to that produced with anti-IgG antisera.
By ELISA, the IgG contained less than 1% IgA and IgM. Purified normal bovine IgG is isolated from bovine serum using fractionation and DEAE chromatography.
Protein concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis the IgG was shown to 1) react with antiserum specific for bovine IgG and the bovine subclasses IgG1 and IgG2, 2) not react with antiserum specific for IgA or IgM, and 3) produce a single precipitin arc with antiserum against bovine serum identical to that produced with anti-IgG antisera.
By ELISA the IgG contained less than 1% IgA and IgM. Purified normal bovine IgG is isolated from bovine serum using fractionation and DEAE chromatography.
Protein concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis the IgG was shown to 1) react with antiserum specific for bovine IgG and the bovine subclasses IgG1 and IgG2, 2) not react with antiserum specific for IgA or IgM, and 3) produce a single precipitin arc with antiserum against bovine serum identical to that produced with anti-IgG antisera.
By ELISA the IgG contained less than 1% IgA and IgM.
Protein concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis the IgG was shown to 1) react with antiserum specific for bovine IgG and the bovine subclasses IgG1 and IgG2, 2) not react with antiserum specific for IgA or IgM, and 3) produce a single precipitin arc with antiserum against bovine serum identical to that produced with anti-IgG antisera.
By ELISA, the IgG contained less than 1% IgA and IgM. Purified normal bovine IgG is isolated from bovine serum using fractionation and DEAE chromatography.
Protein concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis the IgG was shown to 1) react with antiserum specific for bovine IgG and the bovine subclasses IgG1 and IgG2, 2) not react with antiserum specific for IgA or IgM, and 3) produce a single precipitin arc with antiserum against bovine serum identical to that produced with anti-IgG antisera.
By ELISA the IgG contained less than 1% IgA and IgM. Purified normal bovine IgG is isolated from bovine serum using fractionation and DEAE chromatography.
Protein concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis the IgG was shown to 1) react with antiserum specific for bovine IgG and the bovine subclasses IgG1 and IgG2, 2) not react with antiserum specific for IgA or IgM, and 3) produce a single precipitin arc with antiserum against bovine serum identical to that produced with anti-IgG antisera.
By ELISA the IgG contained less than 1% IgA and IgM.
Country of Origin
USA
Additional Product Information
IgG is the primary immunoglobulin protein found in blood/plasma, and functions to neutralize toxins, active complement, immobilizing pathogens and inducing opsonization, and cell-mediated cytotoxicity.
Applications
Used as a positive control in immunoassays. Used as a positive control in immunoassays.