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Primary Antibodies  

Chicken anti-V5 Tag Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog # A190-118A
Citations (10)
Target:
V5 Tag
Applications:
ELISA, ICC, IP, WB
Host:
Chicken
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
Unconjugated (0.1 mg)

A190-118A
$309.00
Qty:
Bulk Request

Product Details

Specifications

Concentration
1 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
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Production Procedures
Chickens were immunized with a synthetic peptide representing amino acid residues 95 to 108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of simion virus 5 conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

The V5 epitope tag is an epitope tag with a 14-residue sequence of GKPIPNPLLGLDST. Derived from the RNA polymerase alpha subunit of simian parainfluenza virus 5, V5 tags can help with detection of proteins in western blot, IP, or microscopic techniques. also especially small and are less likely to interfere with protein function.

Applications

Not all listed applications have been specifically tested by our laboratory. All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.