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Goat anti-GST Tag Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog # A190-121A
Target:
GST Tag
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
Unconjugated (0.1 mg)

A190-121A
$127.00
Qty:

Product Details

Specifications

Concentration
1 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
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Production Procedures
Goats were immunized with Glutathione-S-Transferase (GST) from Schistosoma japonicum. Antibody was isolated by affinity chromatography using GST immobilized on solid support.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

The GST tag is derived from glutathione S-transferase. Its affinity for glutathione makes it ideal for protein purification purposes, but can also be used for WB, IP, and microscopic purposes. The tag is larger than most epitope tags and contributes to solubility of the tagged protein. Further, the tag can also be removed via a proteolytic cleavage if needed.

Applications

Not all listed applications have been specifically tested by our laboratory. All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots are performed using Donkey anti-Goat IgG Heavy and Light Chain Antibody (Cat. No. A50-101P).