Goat anti-Human C-Reactive Protein (CRP) Antibody Biotinylated
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human C-Reactive Protein (CRP). This antibody may cross react with C-Reactive Protein (CRP) from other species. Goats were immunized with purified human C-reactive Protein (CRP) of serum or plasma origin. Antiserum was solid phase adsorbed to ensure specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human C-Reactive Protein. Goats were immunized with purified human C-reactive Protein (CRP) of serum or plasma origin. Antiserum was solid phase adsorbed to ensure specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human C-Reactive Protein (CRP). This antibody may cross react with C-Reactive Protein (CRP) from other species. Goats were immunized with purified human C-reactive Protein (CRP) of serum or plasma origin. Antiserum was solid phase adsorbed to ensure specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human C-Reactive Protein. Goats were immunized with purified human C-reactive Protein (CRP) of serum or plasma origin. Antiserum was solid phase adsorbed to ensure specificity. The antiserum was fractionated and passed over DEAE to yield an IgG fraction.
Total protein was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis, the antiserum reacts specifically with human C-Reactive Protein (CRP). No antibody was detected against other serum proteins.
Additional Product Information
The protein encoded by this gene belongs to the pentraxin family which also includes serum amyloid P component protein and pentraxin 3. Pentraxins are involved in complement activation and amplification via communication with complement initiation pattern recognition molecules, but also complement regulation via recruitment of complement regulators. The encoded protein has a calcium dependent ligand binding domain with a distinctive flattened beta-jellyroll structure. It exists in two forms as either a pentamer in circulation or as a nonsoluble monomer in tissues. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. Elevated expression of the encoded protein is associated with severe acute respiratory syndrome coronavirus 2 infection. [provided by RefSeq, Aug 2020]
Alternate Names
C-reactive protein; C-reactive protein, pentraxin-related; pentraxin 1; PTX1
Applications
Not all listed applications have been specifically tested by our laboratory.
For use in precipitin gel reactions (e.g. immunoelectrophoresis (IEP), double diffusion Ouchterlony (DD) or immunofixation electrophoresis (IFE)).
Optimal working dilutions should be determined experimentally by the investigator.